construction of eukaryotic expression vectors encoding cfp-10 and esat-6 genes and their potential in lymphocyte proliferation
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abstract
background: mycobacterium (m.) bovis is the agent of bovine tuberculosis (tb) in a range of animal species, including humans. recent advances in immunology and the molecular biology of mycobacterium have allowed identification of a large number of antigens with the potential for the development of a new tb vaccine. the esat-6 and cfp-10 proteins of m. bovis are important structural and functional proteins known to be important immunogens. methods: in the current study, the dnas encoding these genes were utilized in the construction of pcdna 3.1+/esat-6 and pcdna3.1+/cfp-10 plasmids. after intramuscular injection of balb/c mice with these plasmids, esat-6 and cfp-10 mrna expression was assessed by rt-pcr. mice were inoculated and boosted with the plasmids to evaluate their effects on lymphocyte proliferation. results: our results indicate the plasmids are expressed at the rna level and can induce lymphocyte proliferation. conclusion: further study is needed to characterize the effect of these antigens on the immune system and determine whether they are effective vaccine candidates against m. bovis.
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Journal title:
reports of biochemistry and molecular biologyجلد ۲، شماره ۱، صفحات ۳۵-۴۱
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